The last decades we have seen considerable technological advances in medicine and electrical engineering, which forced scientists to create complex instruments and sensors. These devices can make medical examinations faster and easier. In this work I would like to present the diversified field of sensor technology and also explain the reasons why optical biosensors, especially the ones based on the principle of surface plasmon resonance (SPR) are in the focus of so many investigations. I would also like to demonstrate the difference between the conventional SPR chips and the nanotechnology based LSPR (LSPR: localized surface plasmon resonance) chips, and take the advantages and disadvantages into consideration. The goal of this work was to optimize the manufacturing parameters of creating gold nanoparticles on silicon and nanoporous aluminium substrates which can perform better than the already existing glass substrates if we would like to transfer our nanoparticles to polymer-based microfluidic systems. I measured the samples with an optical spectrophotometer and also used atomic force microscopy to see how the spectra depend on the geometry of the nanoparticles. I was able to create similar (approximately 50-100 nm diameter, 550 nm absorbance peak) nanoparticles on silicon substrate than the ones we had made on glass with the already existing technology. Besides this, I was able to transfer the nanoparticles from aluminium substrate to PDMS (polydimethylsiloxane) substrate, and also from glass substrate to PMMA (poly(methyl methacrylate)) substrate.