In my thesis transparent microelectrode substrate, Parylene HT was characterized optically and statistically by Two-Photon Excitation Microscopy with fluorescent microbeads.
In this work the properties of the transparent polymer, the technologies used for their optical characterization and the ex vivo experimental processes were presented.
For electrical characterization, the impedance of electrophysiological recording sites was measured by electrochemical impedance spectroscopy (EIS) both before and after the implantation.
The algorithm in MATLAB is able to analyse the intensity profile of the fluorescent pictures, processed by Two-Photon Excitation Microscopy. I participated the optical experiments and then I evaluated the created data by MATLAB. Optical characterization was made by comparing the intensity profiles of two different pictures, one with the transparent polymer used as electrode substrate (Parylene HT), and the other without the substrate.
As a conclusion, the noise and the deformation in the diamter of the microbeads and axons, caused by the presence of Parylene HT was insignificant.