In the last two semesters I made experiments related to microfluidic research in the Department of Electron Devices at Budapest University of Technology and Economics. A thermostable environment over a thermopile-based calorimeter was designed and built. Temperature-sensitive thermocouples were located on a SiN membrane. Two drops with a volume of 1 μl were pipetted onto the membrane by electronically controlled pneumatic valves. The first drop was pc-PAL (L phenylalanine ammonia-lyase) enzyme immobilised on surfaces of MNP (magnetic nanoparticle), the second one was L phenylalanine substrate. After the measurements were carried out at a station which was created for calorimetry analysis, the results were evaluated. Based on them, to isolate clearly the enthalpy created by enzyme reactions was unsuccessful.
Furthermore, spectrophotometric flow-cells were designed and created with 3D printing technology for a Micronit microfluidic chip clamping station. Absorbance and characterization measurements were completed with the developed cells. TRIS (Tris(hydroxymethyl)aminomethane) buffer solution and cinnamic acid were used as reference and standard solutions respectively in the experiments. The results were evaluated, the conclusions were reached. Based on the results it can be stated that the 3D printed flow-cells can be used with high reliability for tracking tests of bioreactions in LoC systems.