The project was based on designing a microfluidic setup to detect Phenylalanine levels in a microfluidic chip using Phenylalanine ammonia-lyase (PAL) enzyme immobilized on to MNPs which are used as biodetector. This was done by analyzing the enzyme activity at different substrate concentrations. Based on the absorbance measurements of the reaction, the enzyme kinetic parameters were determined which provide the reaction rate based on varying conditions. The lab on chip technique with MNPs coated with immobilized enzyme would provide a reliable, lesser time consuming and easier technique for the detection of the Phenylketonuria disease, which is caused by increased level of Phenylalanine in humans. PKU is commonly included in the newborn screening panel of most countries, with varied detection techniques. Untreated children often fail to attain early developmental milestones, develop microcephaly, and demonstrate progressive impairment of cerebral function. In general, however, outcomes for treated PKU patients are good especially with continuous monitoring of the phenylalanine level in the urea.