Investigation of microreactor cells of Lab-on-a-Chip devices with simulation

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Dr. Ender Ferenc
Department of Electron Devices

The goal of the work presented in this thesis is the investigation of some processes taken place in a Lab-on-a-Chip device.

In the device, we perform an enzyme-substrate reaction to detect the phenilyketonuria disease that causes the rise of the L-phenylalanine (L-phe) amino-acid's concentration in the blood. Decomposing the amino-acid to cinnamic-acid and ammonia with PAL enzyme, the quantity of cinnamic acid can be measured. The concentration of the L-phe can be determined by knowing the parameters of the enzyme kinetics and the quantity of the cinnamic-acid.

The microfludic structure consits of chambers with a volume in a micorlitre scale, and several channels that connect the chambers serial. Continuous fluid flow in the system is performed. The enzyme is immbolizized into the surface of nano-sized magnetic particles (MNPs) with a diametre of few hundred nanometres. Medium of magnetic nano particles are transported into the chambers with the fluid flow as a suspension. This medium is positoned in the chambers with external magnetic field. Then, transporting the L-phe substrate in the fluid flow the enzyme-substrate reaction can be performed.

In this thesis, we examine the following processes with numerical simulations:

-Modeling the charging process of the chamber with fluid at different flowrates. Goal of the charging process is not to generate any bubbles, as they can prevent the fluid flow.

-Examining the empty as well as the MNP filled chamber's flow field. Medium of the magnetic nano particles modify the originally empty chamber's flow field.

-Modeling the enzyme-substrate reaction in the chamber.

Numerical simualtions are performed with ANSYS software. For creating the simualtions, results of several measurements with the device is used.


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