Aggregation study and analysis of DJ-1 protein for the development of disease modifying therapeutics

OData support
Supervisor:
Dr. Jobbágy Ákos Andor
Department of Measurement and Information Systems

Due to the aging population, the incidence of neurodegenerative diseases gradually increases nowadays (especially Alzheimer’s disease, Parkinson’s disease), so more and more attention aims at researches in impairment of nerve cells. Mainly because of the loss of the neurons’ function dementia can be observed. By the extended course of these diseases, individuals have to cope with vast emotional and financial burden, eventually there are no any effective therapies for the reduction of the symptoms. The required cost of care and support can reach billions. In 2015 the number of people with dementia was approximately 46.8 million.

In my research I investigate the aggregation of DJ-1 protein and its oxidized variants, in addition the effect of DJ-1 protein on the aggregation of α-synuclein. Understanding the mechanism of aggregation of DJ-1 protein in vitro may provide clues further information for the on-set and the pathogenesis of Parkinson’s disease, hereby developing an effective and targeted therapy.

The problem is complex, because not only the procession of diseases is not fully understood, but also their causes. Cooperation of medical and engineering sciences is needed to discover fully mechanisms of neurodegenerative diseases. After the applied physical-chemical and biophysical methods (dynamic light scattering, transmission electron microscopy, circular dichroism spectroscopy, Native PAGE electrophoresis) and optimization of in vitro experiments, I can describe a suitable model for tracking the aggregation, which can make it possible to simulate brain processes in the future.

Downloads

Please sign in to download the files of this thesis.