Investigation of living cells in flow fields with label-free optical biosensors

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Supervisor:
Dr. Bonyár Attila
Department of Electronics Technology

Nowadays, biosensorics has gotten more attention. We are able to develop various

sensors, which offer high accuracy and specific definition of biological

reactions/processes and diseases.

The main objective of my independent laboratory work was to experimentally test

a specific fluidic cell employed on the surface of a microplate containing optical

waveguide based sensor units. A magnetic mixer positioned at the top of the flow cell

allowed to create a well-controlled rotating fluidic flow over the sensing elements.

Therefore, the effect of fluid flow on living cells adhering or already adhered onto the

sensing elements could be readily investigated in a straightforward and elegant way.

During my project I experimentally characterised the rotation of the magnetic mixer

and possible noises introduced by its motion on the optical biosensor measurements. . I

also rated the nature and extent of the noise during rotation.

The detailed characterization of the flow space created by the mixer, aimed at to

quantify the effect of the fluidic medium on living cells adhering onto the sensor surfaces.

My thesis work contained the definition of a novel problem, was supported by

literature search, involved feasibility tests, planning of experiments, problem solving and

the evaluation of the recorded data.

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