Parameter dependency investigation of enzymatic reactions in Lab-on-a-Chip devices

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Supervisor:
Dr. Ender Ferenc
Department of Electron Devices

Biochemical tests could help us set up a diagnosis, follow the progression of a disease, filter diseases and judge the prognosis. In the last few years with the developement of bioenergetics and biosensors and the broaden the field of its use, a new need has arisen for a simpler, more efficient and reproductable measuring methods among researchers.

The Lab-On-a-Chip is a microfluidic system which compresses the functions of a biological laboratory inside a chip.

The use of magnetic nanoparticles in microfluidic chip gives us many possibility for science. It's adventegous attributions are that different molecules can be binded to its surface and the particles can be separated from the reaction agent easily with the help of a magnetic field.

We are building a microfluidic system where we catch enzyme binded magnetic nanoparticles in the chips chamers with the help of neodinium magnets, flow the substrate solution across the chip and measure the rate of product generation.

The goal was to build up an automatic system, which can be controlled by the user by the help of a controlling software. I planned and made a software which tend to navigate a fluid thermostate supposed to heat the chip. Subsequently it was changed to a Peltier-cell controlled by a Laird controller so I developed the software to be able to navigate the controller too. This program will be inserted in the main software after additional developments.

Enzyme activity depends on the temperature of the reaction agent as well. Almost every enzyme has an optimum for working temperature. In spite of this statement ex situ measurements confirm that binded enzymes have much bigger activity in higher temperatures than free enzymes. We made this measurement in situ in our chip and get similar results as ex situ measurements.

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