Investigation of enzyme coated nanoparticles in Lab-on-a-Chip microcalorimeter device

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Supervisor:
Dr. Ender Ferenc
Department of Electron Devices

In the last few years the medical diagnostics is going on the way of miniaturization. This occasion served the spreading of the point of care devices. The achievements of Lab-on-a-chip technology can give new buoyancy to the growth of these kinds of examinations.

The purpose of our project is to invent a Lab-on-a-chip calorimetric device. While carrying out this we bind enzymes to magnetic nanobeads, we drive them throw microfluidic channels, and there we fix them with external magnets. My role was to test the principles in practice of fixing, rinsing, and washing of the particles how they affect the fluid flow and how the phase changes affect the fixing of the particles. Furthermore how the enzymatic activity changes upon the fixing and washing of the particles.

I found that fast sedimentation of the beads, and the rounded shape of the chambers makes a problem during drive the particles into the channel. That is why it was not possible to inject them into the channel and regain them fully controlled. Also the phase changes made complications because of these malfunctions. In spite of this we gained nice result while fixing the beads in the channel. That was not much affected by the phase changes either.

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