Investigation of protein based biosensors with surface plasmon resonance imaging

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Supervisor:
Dr. Bonyár Attila
Department of Electronics Technology

The „Sensors and Microfluidics” laboratory at the Department of Electronics Technology have been studying biosensors for nearly ten years. Within the framework of a EU FP-6 project a surface plasmon resonance imaging (SPRi) device was built which can be utilize for label-free biosensing. During these years a well-developed, thiol based covalent immobilization method was utilised to bind the receptor molecules on the gold covered SPRi chips, but it has some drawbacks (e.g. impossible to reuse costly SPRi chips). This fact inspired us to investigate new immobilization techniques.

The aim of my work is to find, implement and characterize an immobilization method which would enable the possibilty to regenerate the costly SPRi chips. Based on the results of thorough literature analysis I selected a method which is based on bioaffinity interactions between the biotin-avidin molecules. This method would grant the possibility to regenerate the receptor layer and also versatility in preparing a multi-bioreceptor sensor.

Besides the implementation and characterization of this new and promising method I enhanced the microfluidic system of our SPRi device by developing a new and easy-to-use syringe pump system. I collected information regarding the operation of pump and the maintenance of the SPRi device accessories in the appendix.

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