Development of methods and tools for the investigation of physiological effects of hyperbaric environment

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Supervisor:
Dr. Sántha Hunor
Department of Electronics Technology

The aim of the work presented in this paper is to investigate various methods suitable for the regeneration of surviving cells after, ischaemic conditions. One of these methods is cooling the cells during the ischaemic period. One purpose of the experiments performed is to determine the relationship between reduced temperature and ischeamic injuries . The other method is conditioning the cells with high pressure oxygen. The essence of this method is to justify the assumption that the resistance of cells against ischaemia can be increased with high pressure oxygen therapy. Additionally it was a task to design a system suitable for cooling in vitro cell cultures and perform pilot measurements using the prototype. During the development we successfully built the device, which was able to keep the cell cultures at 4 degree without any regulation at the equilibrium point. Our experiments showed some unexpected results as well after cold ischeamia. The tests showed that the effect of cooling was harmful in case of mesenchymal stem cells, while in case of neural cells the viability increased, as we expected. In terms of the results of hyperbaric conditioning we found that its effect was not significantly harmful in case of normal healthy cells, while in case of cold ischaemic cells the surplus oxygen had beneficial impact. For more accurate interpretation of the results further investigations are necessary. These experiment series can be easily performed by the system created as result of thesis work.

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