Synthesis and investigation of poly(aspartamide) based hydrogel scaffolds

OData support
Supervisor:
Juriga Dávid
SE Biomedical Engineering MSc

The loss or failure of an organ or tissue is one of the most frequent and expensive problem in human healthcare.The number of the successful transplantations is limited by donor shortage or rejection of the implanted organ/tissue [1]. These problems could be elmininated by tissue engineering. In tissue engineering, a new tissue/organ can be constructed in a laboratory using the cells of the patient that would be implanted back to the body. The natural extracellular matrix (ECM) is a complex structure, which is necessary for the cell growth, differentation and migration [2] . The structural components of natural ECM are collagen, elastin, adhesive proteins (fibronectin, laminin) and gylcosaminoglican, with liquid [3]. The chemical composition and mechanical property of the ECM play a major role in appropriate reproduction and differentation of cells. In vitro cell culture requires those materials which have similar properties to the ECM. For this purpose polymer hydrogels might be suitable since these material contain a 3 dimensional polymer network filled up with liquid [4] . They are also called intelligent materials, as they can respond to changes the environment thus creating cell-tissue support communication.

If polymer gels are modified with these materials which are necessary for cell growth, adhesion and 3 dimensional migration it can increase the effectiveness of the procedure. Since the natural ECM mainly contains amino acids in my diploma thesis, I focused to synthesize different poly(amino acid) based gels which could be used as a scaffold.The objectives of my work are the following:

1. Synthesis of poly(aspartic acid) (PASP) anhydride of poly(succinimide) (PSI), and modification of the main polymer with various functional groups and molecules which can increase the cell adhesion, migration and proliferation.

2. Characterization of the chemical structure of the polymers and the gels by FTIR and NMR spectroscopy.

3. Investigating the applicability of the prepared gels as scaffold (viability and morphology) in a collaboration with the Department of OralBiology at Semmelweis University.

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