Virtual fluorescent microscopy to identify genetic deviations

OData support
Supervisor:
Dr. Jobbágy Ákos Andor
Department of Measurement and Information Systems

The revolutionary development of the information technology opens up new opportunities to all areas of medicine. The development of science provides more and more challenges, with which the traditional methods are no longer able to keep up. The microscope’s reports do not contain visual information about the sample, and do not provide for this possibility. The appearance of the digital pathology opens up new opportunities to the diagnostic, the research, and also to the education. The Fluorescence in situ hybridization (FISH) techniques to the fore, furtheremore it ensures the safety of cytogenetic diagnosis, and not only for the metaphase cells but also for the interface cells. The FISH molecular techniqs used in tumor diagnostics for exapmle in the hematology and solid tumors cases, and are also used in the prenatal genetic disorders detection. FISH looks specifically at the one specific gene area of a chromosome only.

The purpose of the investigation:

The biomarkers which labeled with DNA specific fluorescent dye are conquering in the routine diagnostic pathology. Our aim is to present our new developed FISHQuant quantification algorithm and compare the semi-automatid image-processing results with the manually evaluated measurement on the same samples. We response to cnlinilac cases, which identification of genetic variation of the FISH assay is part of the routine diagnostic procedures and which have a high degree of clinical relevance.

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